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PCR, PCRÁ¾·ù, PCR°úÁ¤, PCR±âº»¿ø¸® Á¤¸®·¹Æ÷Æ® / 1. Title 2. Date 3. Introduction #1, PCRÀ̶õ ¹«¾ùÀΰ¡ #2, PCRÀÇ ±âº» ¿ø¸® #3, PCR °úÁ¤ #4, PCRÀÇ ±¸¼º¿ä¼Ò #5, PCRÀÇ Á¾·ù #6, PCRÀÇ ½ÇÇè¹æ¹ý #7, PCRÀÇ ½ÇÇè ÁÖÀÇ»çÇ× #8, PCR ÀÀ¿ë ºÐ¾ß / 1. Title PCR, Áï ÁßÇÕÈ¿¼Ò ¿¬¼â ¹ÝÀÀ(Polymerase Chain Reaction)Àº DNAÀÇ Æ¯Á¤ ºÎºÐÀ» ´ë·®À¸·Î ÁõÆøÇÏ´Â Çõ½ÅÀûÀÎ ±â¡¦ |
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[ÀÚ¿¬°úÇÐ] ÀϹݻý¹°ÇÐ - DNA ÃßÃâ ¹× PCR & PCR °á°ú È®ÀÎ(Àü±â¿µµ¿) / DNA ÃßÃâ ¹× PCR & PCR °á°ú È®ÀÎ(Àü±â¿µµ¿) 1. ½ÇÇè ¸ñÀû(Purpose of experiment) (1) ÁãÀÇ ±ÙÀ°¼¼Æ÷¿¡¼ DNA¸¦ ÃßÃâÇÏ¿© ÁßÇÕÈ¿¼Ò ¿¬¼â ¹ÝÀÀ(polymerase chain reaction, PCR) ½ÇÇè¿¡ ÀÌ¿ëÇÒ product¸¦ ¸¸µç´Ù. (2) Áö³ 11ÁÖÂ÷¿¡ ÃßÃâÇÑ DNAÀÇ PCR°á°ú¸¦ Àü±â¿µµ¿À» ÅëÇØ È®ÀÎÇÑ´Ù. 2. ¹è°æÁö½Ä(Background ¡¦ |
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[ÀÚ¿¬°úÇÐ]½ÇÇ躸°í¼ - PCRÀÇ ¿ø¸®¸¦ ¹è¿ì°í Àü±â¿µµ¿À» ÀÌ¿ëÇÏ¿© DNA band¸¦ È®ÀÎÇغ¸´Â ½ÇÇè[PCR°Ë»ç] / PCR °Ë»ç ½ÇÇè ¸ñÇ¥ DNA¿Í PCR¿¡ ´ëÇÏ¿© ÀÌÇØÇÏ°í Á÷Á¢ ƯÁ¤ À¯ÀüÀÚ¸¦ ÁõÆø ÇØ º»´Ù. ½ÇÇè ÀÌ·Ð DNA(deoxyribonucleic acid) : ÇÙ»ê(nucleic acid)ÀÇ ÀÏÁ¾À¸·Î, ¼¼Æ÷³»¿¡¼ »ý¹°ÀÇ À¯ÀüÁ¤º¸¸¦ º¸°üÇÏ´Â ¹°Áú ÀÌÁß³ª¼±±¸Á¶¸¦ ÀÌ·ç¸ç °¢ ³ª¼±Àº »À´ë¿Í ¿°±â·Î ±¸¼ºµÈ´Ù. »À´ë¡¦ |
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PCR ¹× Àü±â¿µµ¿ (PCR and Agarose Gel Electrophoreisis) / 1. Title 2. Date 3. Object 4. Introduction 4-1. PCR 4-2. Agarose gel electrophoresis 5. Materials & Methods 5-1. Materials 5-2. Methods 6. Results 6-. Results À̹ÌÁö 6-2. Results ºÐ¼® 7. Discussion / 1. Title PCR ¹× Àü±â¿µµ¿Àº ºÐÀÚ »ý¹°ÇÐ ºÐ¾ß¿¡¼ ³Î¸® »ç¿ëµÇ´Â Áß¿äÇÑ ±â¼ú·Î, À¯Àü¡¦ |
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PCR °ú QPCR(real time PCR) / 1. ±âº» PCRÀÇ ÀÌÇØ 2. QPCR(quatitive PCR) = RT PCR(real time PCR) / 1. ±âº» PCRÀÇ ÀÌÇØ ±âº» PCR(Polymerase Chain Reaction)Àº ƯÁ¤ DNA ¼¿À» ¼±ÅÃÀûÀ¸·Î ÁõÆøÇÏ´Â ±â¼ú·Î, 1980³â´ë ÃÊ¹Ý È¦¸Ó½º¿Í ±×ÀÇ µ¿·áµéÀÌ °³¹ßÇÏ¿´´Ù. PCRÀÇ ÁÖµÈ ¸ñÀûÀº ¿øÇÏ´Â DNA Á¶°¢À» ºü¸£°í È¿À²ÀûÀ¸·Î ÁõÆøÇÏ¿© ¿¬±¸³ª Áø´Ü¿¡ È°¿ëÇÒ ¼ö ÀÖ°Ô ÇÏ´Â °ÍÀÌ´Ù. ÀÌ ¡¦ |
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PCR(ÁßÇÕÈ¿¼Ò¿¬¼â¹ÝÀÀ) ½ÇÇè - pcr buffer Á¦ÀÛ / ¥° Purpose(¸ñÀû) ¥± Introduction(¼Ò°³) ¥² Materials & Methods(½ÇÇèÀç·á ¹× ¹æ¹ý) ¥³ Result(°á°ú) ¥´ Discussion(°íÂû) VI Figures(»çÁø, À̹ÌÁö) VII References(Âü°í¹®Çå) / ¥° Purpose(¸ñÀû) PCR(ÁßÇÕÈ¿¼Ò¿¬¼â¹ÝÀÀ) ½ÇÇè¿¡¼ PCR ¹öÆÛ¸¦ Á¦ÀÛÇÏ´Â ¸ñÀûÀº È¿À²ÀûÀÌ°í Á¤È®ÇÑ DNA º¹Á¦¸¦ À§ÇÑ ÃÖÀûÀÇ ¹ÝÀÀ ȯ°æÀ» Á¦¡¦ |
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qPCR(real-time PCR, RT PCR) / 1. Date 2. Title 3. Object 4. Introduction 4.1. Real time PCR 4.2. Real time PCR standard 5. Material & Methods 5.1. Materials 5.2. Methods 5.3. qPCR condition 6. Results 6.1. Standard curve 6.2. qPCR °á°ú¸¦ ÅëÇÑ initial sample(ȯ°æ½Ã·á) ¹× ¹è¾ç sample ³» DNA Á¤·® 7. Discussion 7.1. AOA °á°ú 7.2. AOB °á°ú ¡¦ |
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1) DNA Extraction 2) PCR & DNA purification 3) Real-Time PCR 1Â÷ 4) plasmi.. / 1) DNA Extraction 2) PCR & DNA purification 3) plasmid extraction 4) Real-Time PCR ¥±. ½ÇÇè Àç·á ¹× °úÁ¤ / 1) DNA Extraction 2) PCR & DNA purification 3) Real-Time PCR 1Â÷ 4) plasmid extraction 5) Real-Time PCR 2Â÷ ¥². °á·Ð ¹× °íÂû |
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DNA ÃßÃâ ¹× PCR, PCR °á°ú È®ÀÎ(Àü±â¿µµ¿) / 1. ½ÇÇè ¸ñÀû(Purpose of experiment) 2. ¹è°æÁö½Ä(Background knowledge) 3. °¡¼³ ¼³Á¤ (Setting up hypothesis) 4. ½ÇÇè(Experiment) 5. °á°ú(Result) 6. °íÂû(Discussion) / 1. ½ÇÇè ¸ñÀû(Purpose of experiment) DNA ÃßÃâ ¹× PCR, PCR °á°ú È®ÀÎ(Àü±â¿µµ¿) ½ÇÇèÀÇ ¸ñÀûÀº »ý¹°ÇÐÀû »ùÇÿ¡¼ À¯Àü¹°ÁúÀÎ DNA¸¦ È¿°úÀûÀ¸·Î ºÐ¸®ÇÏ¡¦ |
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[ÀÎõ´ë »ý¸í°øÇнÇÇè1 A£«] DNA ISOLATION, PCR,16S rRNA gene sequencing , phylogenetic tree analysis ½ÇÇè°á°ú º¸°í¼ / 1. Abstract 2. Information 3. Materials and Methods 4. Result 5. Discussion 6. Conclusion 7. Reference / 1. Abstract º» ¿¬±¸¿¡¼´Â ÀÎõ´ëÇб³ »ý¸í°øÇнÇÇè1 ¼ö¾÷ÀÇ ÀÏȯÀ¸·Î DNA ºÐ¸®, PCR(ÁßÇÕÈ¿¼Ò ¿¬¼â ¹ÝÀÀ), 16S rRNA À¯ÀüÀÚ ¿°±â¼¿ ¡¦ |
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