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�뼚 �떎�뿕�젣紐� : GPC (Gel Permeation Chromatogrphy)
�뼚 �떎�뿕�씪�옄 :
�뼚 �떎�뿕紐⑹쟻 : GPC�쓽 �썝由ъ�� �듅吏뺤쓣 �궡�렣蹂닿퀬, �궗�슜踰뺤쓣 �씡�엳硫� 洹멸쾬�쓽 寃곌낵移섎�� �빐 �꽍�븳�떎.
�뼚 湲곕낯�씠濡�
?? GPC �꽌濡�
GPC (gel permeation chromatography)�뒗 �뼱�뼡 湲� 而щ읆�븞�뿉 媛�援먮맂 怨좊텇�옄 (gel)濡� 異⑹쭊 �떆�궎怨�, �씠 而щ읆�븞�쑝濡� 怨좊텇�옄�슜�븸�쓣 �닾怨� (permeation)�떆耳� 而щ읆�븞�뿉�꽌 怨좊텇�옄媛� �겕湲곗뿉 �뵲�씪 遺꾨━�맆 �닔 �엳�룄濡� 怨좎븞�맂 遺꾨━踰뺤씠�떎. �돺寃� 留먰븯硫�, 遺꾩옄�웾�씠 �겙 臾쇱쭏�씠 癒쇱�� �쑀異쒕릺怨�, �옉��� 遺꾩옄�웾臾쇱쭏�씠 �뒓由ш쾶 �굹�삤�룄濡� �븯�뿬, 怨좊텇�옄媛� 而щ읆�궡遺��뿉�꽌 �뼹留덈굹 癒몃Т�뒗媛� �븯�뒗 �떆媛� (retention time)�쓣 痢≪젙�븯�뿬 怨좊텇�옄�쓽 遺꾩옄�웾�쓣 �궛異쒗븷 �닔 �엳�룄濡� �븳 寃껋씠�떎. 怨좊텇�옄�쓽 �겕湲곕퀎濡� 遺꾨━�븳�떎怨� �빐�꽌 SEC (size exclusion chromatography) �씪怨좊룄 遺�瑜몃떎.
GPC (Gel Permeation Chromatogrphy)�뒗 怨좊텇�옄�쓽 遺꾩옄�웾(M.W.)怨� 遺꾩옄�웾 遺꾪룷(M.W.D.)瑜� 寃곗젙�븯�뒗 媛��옣 湲곕낯�쟻�씤 諛⑸쾿�쑝濡� �슜異쒕쾿�뿉 �쓽�븳 �븸�긽-怨좎긽�삎�쓽 �븸泥� �겕濡쒕쭏�넗 洹몃씪�뵾�쓽 �씪醫낆쑝濡� �씠誘� 遺꾩옄�웾�쓣 �븣怨좎엳�뒗 �몴以��떆猷�(standard sample)��� �깉濡쒖슫 �떆猷�(unknown sample)�쓽 �긽����쟻�씤 �룊洹� 遺꾩옄�웾怨� 遺꾩옄�웾�쓽 遺꾪룷瑜� 寃곗젙�븯�뒗�뜲 �궗�슜�맂�떎. ���遺꾩옄 臾쇱쭏怨� �떖由� 怨좊텇�옄 臾쇱쭏��� 以묓빀�냽�룄�쓽 李⑥씠�뿉 �쓽�븳 �궗�뒳 湲몄씠�쓽 遺꾪룷(遺꾩옄�웾 遺꾪룷) 諛� 以묓빀�룄(DP)媛� 議댁옱�븯寃� �릺�뒗�뜲 怨좊텇�옄�쓽 遺꾩옄�웾怨� 遺꾩옄�웾 遺꾪룷�뒗 怨좊텇�옄 臾쇱쭏�쓽 �뿭�븰�쟻 �꽦吏�, �뿴�쟻 �꽦吏� 洹몃━怨� 湲곌퀎�쟻 �꽦吏덉뿉 �겙 �쁺�뼢�쓣 誘몄튂寃� �릺怨� 理쒖쥌�쟻�쑝濡� 媛�怨듭꽦�쓽 李⑥씠瑜� �굹����굹寃� �븳�떎.
GPC�쓽 媛��옣 �겙 �옣�젏��� �떎�뿕 以�鍮꾧낵�젙�씠 鍮꾧탳�쟻 媛꾨떒�븯怨� 痢≪젙 二쇨린媛� 吏㏐퀬 �긽����쟻�씠吏�留� 誘우쓬吏곹븳 寃곌낵瑜� �뼸�쓣 �닔 �엳�쑝硫� �옱�쁽�꽦�씠 媛��뒫�븯�떎�뒗 寃껋씠�떎. GPC瑜� �넻�븳遺꾩옄�웾怨� 遺꾩옄�웾 遺꾪룷 痢≪젙��� 鍮꾟��(�깮�왂)
1) GPC 而щ읆
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怨좊텇�옄 怨듯븰 - GPC [Gel Permeation Chromatogrphy] 遺꾩꽍�씠濡�(2).hwp
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